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Cryomacroscopy of Vitrification:

Selected Experiments on DP6 and VS55

The objective for this web page is to provide a selection of images of physical events such as crystallization and vitrification, and to serve as an appendix for the following two publications:

1. Rabin, Y., Taylor, M.J., Walsh, J.R., Baicu, S., Steif, P.S. (2005): Cryomacroscopy of vitrification, Part I: A prototype and experimental observations on the cocktails VS55 and DP6, Cell Preservation Technology, 3(3):169-183 Article (PDF 1.3MB)

2. Steif, P.S., Palastro, M., Wen, C.R., Baicu, S., Taylor, M.J., Rabin, Y. (2005): Cryomacroscopy of vitrification, Part II: Experimental observations and analysis of fracture formation in vitrified VS55 and DP6, Cell Preservation Technology, 3(3):184-200 Article (PDF 1.6MB)

Related work: Cryomacroscopy of fracture formation in vitrified thin films of cryoprotectants

Cooling Rate	Temperature Range
H1	-40⁰C to -100⁰C
H2	-100⁰C to -130⁰C
H3	-130⁰C to -100⁰C
H4	-100⁰C to -40⁰C

Exp.	H1 ⁰C/min	H2 ⁰C/min	H3 ⁰C/min	H4 ⁰C/min	Tmin  ⁰C
DP6 #41	100.0	106.0	-14.0	-300.0	-156.6	Fractures during cooling followed by recrystallization
DP6 #40	97.3	100.0	-26.5	-400.0	-161.1	Fractures during cooling followed by recrystallization
DP6 #39	80.0	81.8	-26.0	-360.0	-193.7	Fractures during cooling followed by recrystallization
DP6 #31	32.7	31.0	-6.9	-3.5	-158.1	Fractures during cooling followed by recrystallization
DP6 #55	21.2	19.2	-6.1	-8.7	-146.1	Fractures during cooling followed by recrystallization
DP6 #32	17.4	16.1	-4.4	-2.3	-150.6	Fractures during cooling followed by recrystallization
DP6 #54	16.7	14.2	-47.4	-171.4	-142.7	Fractures during cooling (no rewarming above -100⁰C)
DP6 #34	6.1	3.4	-13.2	-9.8	-129.9	Fractures during cooling followed by recrystallization
DP6 #74	4.8	1.1	-18.7	-277.0	-129.6	Fractures during cooling followed by recrystallization
DP6 #36	11.5	7.6	-15.0	-10.0	-136.1	Vitrification followed by recrystallization
DP6 #35	4.4	2.1	-14.3	-9.6	-133.2	Vitrification followed by recrystallization
DP6 #42	2.4	1.5	-14.3	-40.0	-135.7	Vitrification followed by recrystallization
DP6 #43	14.3	11.8	-18.6	-46.2	-133.0	Crystallization followed by recrystallization
DP6 #93	8.4	5.1	-12.1	-29.8	-131.4	Crystallization followed by recrystallization
DP6 #94	7.2	3.4	-13.9	-29.4	-130.6	Crystallization followed by recrystallization
DP6 #82	5.8	2.8	-15.6	-50.7	-128.1	Crystallization followed by recrystallization
DP6 #33	3.5	1.5	-12.6	-9.1	-129.2	Crystallization followed by recrystallization
VS55 #69	5.3	0.9	-15.5	-30.5	-130.6	Fractures during cooling followed by recrystallization
VS55 #60	3.1	0.5	-12.8	-19.0	-131.0	Fractures during cooling followed by recrystallization
VS55 #86	14.3	10.0	-18.7	-53.4	-131.9	Vitrification followed by recrystallization
VS55 #71	7.9	4.1	-16.7	-3.5	-130.7	Vitrification followed by recrystallization
VS55 #101	3.8	2.6	-6.1	-13.0	-131.0	Vitrification followed by minor recrystallization
VS55 #107	3.2	1.9	-5.5	-10.4	-131.1	Vitrification followed by minor recrystallization

This research has been supported, in part, by the National Heart Lung and Blood Institute, NIH Grant # 1R01HL069944

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