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Cryomacroscopy of Fracture Formation

in Vitrified Thin Films of Cryoprotectants

 

The objective for this web page is to serve as an appendix and provide a selection of images on fracture formation for:

 

Rabin, Y., Steif, P.S., Hess, K.C., Jimenez-Rios, J.L., Palastro, M.C. (2006): Fracture formation in vitrified thin films of cryoprotectants. Cryobiology, 53:75-95 Article (PDF 0.9MB)

 

Related work: Cryomacroscopy of vitrification and fracture formation

 

Experimental Setup

 

 

 

Selected Experimental Results

np    number of experiments

nf     number of fractured samples

Tf     temperature at fracture 

ΔTf  maximum temperature difference at fracture

H1    cooling rate between -120°C and -150°C

H2    cooling rate between Tf-10°C to Tf+10°C

 

CPA

Sample Carrier

Muscle Segments

Exp. # or

nf / np

Tf, °C

ΔTf, °C

H1, °C/min

H2, °C/min

6.5M DMSO

None

-

#2

-145.9

1.5

35.9

44.8

#3

-143.8

1.2

11.3

19.5

#12

-142.2

1.2

6.5

9.6

8/8

-143.7±1.9

1.5±0.7

-

-

None

+

#3

-142.9

2.1

49.0

47.4

#6

-140.7

1.2

7.6

10.7

6/6

-142.3±1.2

1.7±0.8

-

-

7.05M DMSO

None

-

#2

-140.6

3.4

49.1

47.1

#4

-141.6

1.7

8.1

12.7

#5

-142.7

1.5

42.7

37.6

#11

-139.6

1.3

6.2

7.7

11/11

-141.7±1.9

1.8±0.7

-

-

None

+

#3

-143.2

3.1

47.8

61.2

#5

-141.9

0.7

6.5

13.3

5/5

-142.2±1.2

2.0±1.1

-

-

1 mm Glass

-

#10

-143.1

9.0

60

-

#14

-145.6

4.6

5 ... 78

-

6/6

-145.2±2.2

6.7±1.9

-

-

150 μm Glass

-

#1

-157.9

3.8

78.7

10.4

#5

-156.7

5.6

63.8

12..43

#7

-150.7

2.0

5.0

12.1

9/9

-159.1±5.4

3.1±1.4

-

-

120 μm Plastic

-

#13

-165.0

1.6

56..72

25..50

4/10

-164.3±3.0

3.0±1.8

-

-

30 μm Plastic

-

#6

-146.7

2.0

44.0

73.0

3*/7

-144.0 ± 3.2

3.6±1.7

-

-

8M DMSO

None

-

#8

-140.9

1.8

33.9

34.2

#9

-139.6

2.0

44.2

43.8

#10

-138.9

0.6

5.6

7.7

#11

-140.6

1.0

7.8

12.7

10/11

-141.1±1.7

1.7±0.9

-

-

None

+

#4

-138.2

2.3

54.6

48.1

#5

-138.3

0.6

5.4

8.1

6/6

-138.0±0.8

1.4±0.7

-

-

8.4M DMSO

None

-

#6

-140.9

0.6

5.5

6.6

#7

-142.1

0.9

1.9

1.1

#11

-141.2

2.9

47.7

49.4

#13

-139.8

0.9

6.0

12.5

12/12

-142.8±3.5

1.5±0.8

-

-

None

+

#1

-137.3

2.6

56.7

51.5

#5

-139.4

1.0

5.9

7.3

#6

-139.6

0.4

4.8

6.6

6/6

-138.5±1.8

1.5±0.9

-

-

VS55

None

-

#2

-138.1

1.7

45.1

40.7

#4

-134.0

1.1

12.6

14.1

#14

-134.0

2.1

51.2

43.1

#15

-133.0

2.1

37.9

33.4

#17

-133.0

0.7

10.7

10.4

17/18

-134.7±2.1

1.4±0.6

-

-

None

+

#4

-138.5

4.2

70.0

61.7

#5

-135.9

4.7

98.8

85.7

#6

-131.4

2.1

48.1

40.0

#7

-136.7

2.6

55.9

50.0

10/10

-135.5±2.7

2.9±1.0

-

-

1 mm Glass

-

#12

-126.2

3.4

77.7

57.4

#14

-153.9

3.5

73.0

39.0

4/6

-144.8±13.0

3.4±0.7

-

-

150 μm Glass

-

#3

-152.7

4.1

49.5

27.5

8/10

-158.6±9.6

4.6±2.1

-

-

120 μm Plastic

-

0/3

-

-

-

-

30 μm Plastic

-

#8

-135.1

3.3

-

-

4/5

-134.0±1.0

3.0±0.2

-

-

DP6

None

-

#5

-137.1

3.5

73.2

60.9

#7

-131.3

3.0

69.7

58.9

#8

-131.6

3.5

78.5

61.7

6/6

-133.2±3.2

3.1±0.6

-

-

None

+

0**/6

-

-

-

-

1 mm Glass

-

#6

-137.0

14.4

86.0

-

#7

-136.2

18.2

96.0

-

#16

-142.2

5.7

88.0

-

6/7

-137.5±6.9

10.6±5.9

-

-

150 μm Glass

-

#2

-156.6

3.6

102.9

-

#10

-162.3

6.3

109.6

-

7/7

-160.7±6.6

4.4±2.1

-

-

120 μm Plastic

-

#1

None

None

-

-

#2

None

None

-

-

#13

-165.0

-

104.0

-

3/7

-154.5±10.1

5.0±1.3

-

-

30 μm Plastic

-

#4

None

None

100.0

-

#9

-155.8

-

79.0

67.0

3/8

-155.8±0.7

3.3±0.7

-

-

* Large temperature gradients

** All samples crystallized

 

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This research has been supported, in part, by the National Heart Lung and Blood Institute, NIH Grant # 1R01HL069944

 

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