Feedback for Multiple Choice Quiz 11

  1. Specificity, the preference for binding one molecule over all others, is due to the complementarity between the molecule and its protein binding site. The complementarity, in turn, is a result of the residues in the binding site.

  2. Look at one of the Scatchard plot graphs in the lecture notes.

  3. All of the parameters except for enthalpy are directly related to one another and interconvertable. (Enthalpy is determined by measuring the temperature dependence of an equilibrium constant.)

  4. Like DNP, Tyr and Phe have a similar aromatic phenyl moiety.

  5. A competing amino acid would lower the apparent affinity. Therefore the Kd would increase from 10-6
    6. to (the only numerically possible answer provided) 10-5 M.

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