Feedback for Multiple Choice Quiz 20b
- The key here is that SDS binds to and denatures proteins to form negatively charged particles of roughly equal charge:mass; hence they separate based soley on mass.
- At pH = 7, histone are positively charged and myoglobin is negatively charged.
- SDS-PAGE yields the monomer Mr; gel filtration yields the native Mr. Both are required to determine the quaternary structure.
- Equivalently: "Larger proteins enter the beads less readily and smaller proteins elute last", i.e. each follows from the other.
- These four statements are also an explanation for the the correct answer to question 9.
All of the correct answers have a green check mark.
Incorrect answers have the "red-lit" button next to your choice.