Feedback for Multiple Choice Quiz 27 #2
- Pyruvate kinase uses ADP as substrate along with PEP to form ATP + pyruvate. Glucokinase and hexokinase both catalyze the phosphorylation of glucose using ATP as substrate. Phosphofructokinase catalyzes the phosphorylation of F-6-P using ATP as substrate. Adenylate kinase is not part of the glycolysis pathway.
- The other four choices all have relatively large -dG's. See Campbell, table 17.1 for details.
- The conformational change that occurs on gluccose binding prevents the hydrolysis of ATP, i.e. the futile "phosphorylation of water". (The reverse of the induced fit conformational change occurs when glucose-6-phosphate is released.)
- One pair of electrons is removed from glyceraldehyde-3-phosphate to form NADH and 1,3-bisphosphoglycerate (step 6). This reaction occurs twice for each glucose. Hence, 2*2 = 4 electrons total/glucose.
- Regeneration of NAD+ is essential for anaerobic glycolysis. Ethanol is produced by yeast, not in the human liver. NADH is used to make ATP in mitochondria under aerobic conditions.
- The human RBC has no organelles and relies on glycolysis for its ATP. Most common bacteria can live under aerobic and anaerobic conditions.
All of the correct answers have a green check mark.
Incorrect answers have the "red-lit" button next to your choice.