Example I
(cont.): Quantitative Analysis Reveals the
Shape of DNA-PK : Ku Complexes Bound to DNA
In contrast, footprints of DNA-PK
bound to DNA in the
presence of Ku are larger, indicating that both proteins form a complex in which they
are located adjacently to each other.
Quantitative Analysis
of Shapes of Individual Proteins and their Complexes
Effective
apparent area (volume/height) provides the “footprint” of a molecule on the
surface.
Footprints of unbound and DNA-bound
DNA-PK in the samples
to which no Ku was added are indistinguishable.
M. Yaneva,T. Kowalewski, M. Lieber EMBO Journal (1997)