Extinction Coefficient of p-nitrophenylate (pNP)

A. Measuring the extinction coefficient of the product following these steps:

  1. Change the concentration of the product, select 4-5 different values.
  2. Obtain the absorbance measurement
  3. Plot absorbance (y) versus concentration (x) and obtain the best fit of the line.
  4. The slope is the molar extinction coefficient.

Note:

  1. Your instrument cannot read absorbance values greater than 2.
  2. There is error in your readings, so select concentrations that give you some values between 1 and 1.5 A
  3. The wavelength for these measurements is 400 nm.
1. Enter a value for [pNP] in this box (Note units are uM):
[pNP] = uM
2. For the above value of [P], Calculate Absorbance
A =
    (Each calculated value has a small "experimental error" added to it.)

10.9.2020