Enzyme Kinetics - Effect of Temperature

Instructions:

  1. Select values of ETot and [S] that will give absorbances less than 2 for all temperatures. The rate will increase as the temperature increases.
  2. Keeping the enzyme concentration and substrate fixed, vary temperature from 20C to 90C and measure product formation.
  3. Use an extinction coefficient of 18,300 M-1cm-1 to convert the absorption to product.
  4. Plot the amount of product produced versus temperature.

Note that your instrument cannot read absorbance values greater than 2.

1. Enter a value for E_total in this box:
ETOT = nM
2. Enter a value for [S] in this box:
[S] = mM
3. Enter a value for temperature in this box:
T(C) =
4. For the above value of pH, Calculate Absorbance/time
A/sec =
    (Each calculated value has a small "experimental error" added to it.)

10.9.2020