Chymotrypsin Substrate Specficity


        
 
    		 

        
 
   
Instructions:
  • Loading Different Substrates:
    • Clicking on "Phe" loads the structure with Phe as the product, i.e. after cleavage.
    • Clicking on "Tyrosine" loads the structure with tyrosine as the product
    • Clicking on "Tryptophan" loads the structure with tryptophan as the product.
  • Clicking on "Simple View" shows the backbone atoms, the product (Ala-X), X=Phe, Tyr, or Trp. in thick sticks + spheres for atoms, and the sidechains of some important residues on the enzyme in thinner sticks.
  • "Add specificity pocket" will display additional residues in the specificity pocket that interact with the sidechain of the substrate.
  • +/- Sub will add and remove the product.
  • +/- Surf. on Sub will add or remove a gray surface on the product (be patient).
  • +/- Surface on triad will place a yellow surface over the three residues in the catalytic triad.
Note:Labeling Atoms:

The simplist method is to place the cursor over the atom and a label will pop-up in a few seconds. If you want a permament label, you can do the following:

  1. Depress the right mouse button to get the pop-up menu.
  2. Select 'Set Picking'
  3. Select 'Label'
Now when you click on an atom, the label will appear in the Jmol window, next to the atom.