Biochemistry I Fall Term   Answer Sheet     Name:

Ligand Binding: Problem Set#4     The ligand used was:
Given in the problem:
1) IgG mg/mL =
2) Mr =
Calculated [IgG] =       mM.
What was found in the experiment:
Ltotal
(mM)		 Lfree
(mM)		 Lbound
(mM)		     n
  		n/[L]
(mM-1)		 
-

-

-

-

-

-

-

-
 
Graph of the calculated results:

The Kd =
The stoichiometry, n =

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10.1.04