The course covers an introduction to the basic molecular techniques used in modern biology research. These are discussed against a background of the molecular and genetic analysis of prokaryotic microorganisms with particular attention to certain selected topics. Among the topics covered are: the transcriptional and translational regulation of gene expression at the molecular level in Escherichia coli, the use of prokaryotic replicons in molecular cloning, and the principles of self-assembly and catalyzed assembly of virus particles.